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Cloning and functional research of Arp2/3-P40/ARPC1 subunit of Sf9 cells

Han, S.-L.; Mu, J.-F.; Zhang, Y.-L.; Chen, X.-W.; Wang, Y.; Li, L.-L.

Bing du Xue Bao 28(6): 601-608

2012

ISSN/ISBN: 1000-8721
PMID: 23367557
Document Number: 658658
The baculovirus-induced actin polymerization is mainly associated with the virus nucleocapsid protein P78/83, which is homologous with WASP proteins that can activate Arp2/3 complex and induce the actin polymerization. In order to explore the role of Arp2/3 complex in the baculovirus replication, the P40 subunit of Arp2/3 complex from Sf9 (Spodoptera frugiperda 9) cell line was cloned and characterized. Immunofluorescent microscopy assay indicated that P40 was recruited to the inner-side of nuclear membrane during virus infection, which was in accordance with nuclear F-actin distribution in virus-infected cells as documented in our previous research, suggesting P40 could be used to track Arp2/3 complex subcellular distribution changes during virus infection. In addition, co-immunoprecipitation assay demonstrated that P40 interacted with P78/83 only in virus-infected cells, suggesting that actin polymerization induced by P78/83-Arp2/3 complex during baculovirus infection was regulated by some unidentified virus factors.

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