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Location of functional regions of acetylcholine receptor alpha-subunit by site-directed mutagenesis

Mishina, M.; Tobimatsu, T.; Imoto, K.; Tanaka, K.; Fujita, Y.; Fukuda, K.; Kurasaki, M.; Takahashi, H.; Morimoto, Y.; Hirose, T.

Nature 313(6001): 364-369

1985

ISSN/ISBN: 0028-0836
PMID: 3838201
Document Number: 247781
The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with alpha-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the alpha-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.

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Fraser, C.M.; Wang, C.D.; Robinson, D.A.; Gocayne, J.D.; Venter, J.C. 1989: Site-directed mutagenesis of m1 muscarinic acetylcholine receptors: conserved aspartic acids play important roles in receptor function Molecular Pharmacology 36(6): 840-847
Wang, C.D.; Buck, M.A.; Fraser, C.M. 1991: Site-directed mutagenesis of alpha 2A-adrenergic receptors: identification of amino acids involved in ligand binding and receptor activation by agonists Molecular Pharmacology 40(2): 168-179
Xu, D.; Jiang, C.; Shi, Y.; Yu, J.; Sun, L.; Fan, P.; Liu, X. 1993: Structure-function studies of the C-terminal alpha-helix of human interleukin-2 by site-directed mutagenesis Chinese Journal of Biotechnology 9(4): 229-234
Alexenko, A.P.; Izotova, L.; Strongin AYa 1990: Reconstruction of an epitope capable of binding murine monoclonal antibodies NK2 within the sequence of human leukocyte interferon alpha F by site-directed mutagenesis Biochemical and Biophysical Research Communications 169(3): 1061-1067
Duvoisin, R.M.; Heinemann, S.F. 1993: Transcription control elements of the rat neuronal nicotinic acetylcholine receptor subunit alpha 3 Brazilian Journal of Medical and Biological Research 26(2): 137-150
Weissenhorn, W.; Chen, Y.H.; Riethmüller, G.; Rieber, E.P.; Weiss, E.H. 1992: VH-related idiotopes detected by site-directed mutagenesis. A study induced by the failure to find CD4 anti-idiotypic antibodies mimicking the cellular receptor of HIV Journal of Immunology 149(4): 1237-1241
Chu, C-Jing.; Yang, Y-Chun.; Wei, J-Xue.; Zhang, L. 2011: Association of nicotinic acetylcholine receptor subunit alpha-4 polymorphisms with smoking behaviors in Chinese male smokers Chinese Medical Journal 124(11): 1634-1638
Horiuchi, T.; Macon, K.J.; Engler, J.A.; Volanakis, J.E. 1991: Site-directed mutagenesis of the region around Cys-241 of complement component C2. Evidence for a C4b binding site Journal of Immunology 147(2): 584-589
Alekseev, T.A.; Dergousova, N.I.; Shibanova, E.D.; Azeeva, E.A.; Kriukova, E.V.; Balashova, T.A.; Dubovskiĭ, P.V.; Aesen'ev, A.S.; Tsetlin, V.I. 2003: 5-fluoro-tryptophan-containing N-terminal domain of the alpha-subunit of the Torpedo californica acetylcholine receptor: preparation in E. coli and 19F NMR study Bioorganicheskaia Khimiia 29(4): 384-390
Shibuya, N.; Kanazawa, H.; Uyemura, K. 1988: Antibody against synthetic peptide corresponding to alpha-subunit residues 128-142 of nicotinic acetylcholine receptor (AChR): the significance in myasthenia gravis (MG) Rinsho Shinkeigaku 28(3): 315-319
Hara, T.; Tanaka, T.; Kato, H.; Nishioka, T.; Oda, J. 1995: Site-directed mutagenesis of glutathione synthetase from Escherichia coli B: mapping of the gamma-L-glutamyl-L-cysteine-binding site Protein Engineering 8(7): 711-716
Piechocki, M.P.; Hines, R.N. 1994: Oligonucleotide design and optimized protocol for site-directed mutagenesis Biotechniques 16(4): 702-707
Kontis, K.J.; Goldin, A.L. 1993: Site-directed mutagenesis of the putative pore region of the rat IIA sodium channel Molecular Pharmacology 43(4): 635-644
Wang, L.M.; Geihl, D.K.; Choudhury, G.G.; Minter, A.; Martinez, L.; Weber, D.K.; Sakaguchi, A.Y. 1989: Site-directed deletion mutagenesis using phagemid vectors and genetic selection Biotechniques 7(9): 1000-6 1008-10
Koyama, T.; Obata, S.; Osabe, M.; Saito, K.; Takeshita, A.; Nishino, T.; Ogura, K. 1994: Thermostable farnesyl diphosphate synthase of Bacillus stearothermophilus: crystallization and site-directed mutagenesis Acta Biochimica Polonica 41(3): 281-292
Loewenstein-Lichtenstein, Y.; Glick, D.; Gluzman, N.; Sternfeld, M.; Zakut, H.; Soreq, H. 1996: Overlapping drug interaction sites of human butyrylcholinesterase dissected by site-directed mutagenesis Molecular Pharmacology 50(6): 1423-1431
Mikulinskaia, G.V.; Taran, S.A.; Skoblov, I.S.; Feofanov, S.A. 2013: Investigation of active center of deoxynucleoside monophosphate kinase of bacteriophage T5 by site-directed mutagenesis Bioorganicheskaia Khimiia 39(6): 680-692
Gao, W.; Dai, S.; Liu, Q.; Xu, H.; Bai, Y.; Qiao, M. 2010: Effect of site-directed mutagenesis of citB on the expression and activity of Bacillus subtilis aconitase Mikrobiologiia 79(6): 774-778
Guillerm, C.; Lellouche, F.; Darnige, L.; Schandelong, A.; Geffroy, F.; Dorval, I. 1996: Rapid detection of the G1691A mutation of coagulation factor V by PCR-mediated site-directed mutagenesis Clinical Chemistry 42(2): 329
Cremonesi, L.; Seia, M.; Magnani, C.; Ferrari, M. 1991: Rapid detection of 1717-1G----A mutation in CFTR gene by PCR-mediated site-directed mutagenesis Clinical Chemistry 37(11): 1967