Sequence analysis of the phosphoprotein gene of peste des petits ruminants virus of Chinese origin
Bao, J.-y.; Zhao, W.-j.; Li, L.; Wang, Z.-l.; Wu, G.-z.; Wu, X.-d.; Liu, C.-j.; Wang, Q.-h.; Wang, J.-w.; Liu, Y.-t.; Li, J.-m.; Wang, Y.-l.
Bing du Xue Bao 27(1): 26-33
2011
ISSN/ISBN: 1000-8721 PMID: 21462503 Document Number: 654741
The nucleotide sequences of P gene from a field strain of peste des petits ruminants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The P gene is 1,655 nucleotides long with two overlapping open reading frames (ORFs). The first ORF is 1530 nucleotides long and would produce P protein of 509 amino acid residues. The second ORF is 534 nucleotides long and would produce C protein of 177 amino acid residues. The first ORF produces a second mRNA transcript of 897 nucleotides long with an extra G nucleotide at position 751. Translation from this mRNA would produce V protein of 298 amino acid residues. The nucleotide and deduced amino acid sequence were compared with the homologous region of other PPRV isolates. At the amino acid level, the "China/Tib/Gej/07-30" shares homology of 86.10%-97.3%, 84.3%-94.9%, and 82.9%-96.3% for P, C, and V proteins respectively. Several sequence motifs in the P genes were identified on the basis of conservation in the PPRVs and the morbilliviruses.