Pectin substances of the callus culture of Silene vulgaris (M.) G
Giunter, E.A.; Ovodov, I.S.
Prikladnaia Biokhimiia i Mikrobiologiia 47(1): 90-94
2011
ISSN/ISBN: 0555-1099 PMID: 21442925 Document Number: 648088
Pectin-protein fraction SVC was isolated from the callus culture of the bladder campion (Silene vulgaris). The main components in it were residues of D-galacturonic acid, galactose, arabinose, rhamnose, and protein. Using ion-exchange chromatography, ultrafiltration, and acid and enzymatic hydrolysis, it was shown that SVC contained a mixture of molecules of linear pectin, branched pectin polysaccharide, and pectin-protein polymer. A fragment of the linear chain of galacturonan amounted to more than half of the entire carbohydrate silenan chain. The branched area of the macromolecule is represented by rhamnogalacturonan I. The pectin-protein polymer consisted mainly of protein and weakly branched pectin fragments with molecular mass of more than 300 kDa.