Reproduction of Pratylenchus brachyurus on Soybean Callus Tissue: Effects of Culture Age and Observations on Anhydrobiosis

Koenning, S.R.; Schmitt, D.P.

Journal of Nematology 18(4): 581-582

1986


ISSN/ISBN: 0022-300X
PMID: 19294230
Document Number: 280697
18-month-old cultures yielded the greatest number of nematodes (100 000/tube) but only 33% were active and looked healthy. Inoculum was best collected, 4-6 months after inoculation when all nematodes were active. Anhydrobiotic nematodes were observed in coiled and in straight shapes, packed together in a parallel fashion.

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