Studies on the medicinal properties of Solanum chrysotrichum in tissue culture: I. Callus formation and plant induction from axillary buds
Villarreal, M.L.; Muñoz, J.
Archivos de Investigacion Medica 22(2): 127-133
1991
ISSN/ISBN: 0066-6769 PMID: 1819987 Document Number: 383157
A tissue culture method is described for micropropagation and callus formation from Solanum chrysotricum axillary bud explants in Murashige and Skoog's (MS) medium, supplemented with various growth regulators. Induction of rooted plants were initiated only when indol-3 acetic acid (IAA) was present as an auxin in combination with either of two cytokinins: kinetin (KN) or benzyladenine (BA); however, the combination of IAA (0.1 mg.lt.-1) + BA (0.2 mg.lt.-1) was found to be best suited for morphogenesis purposes. Alternatively, callus tissue formation was influenced in presence of naphthalene acetic acid; which in combination with kinetin (NAA 0.1 mg.lt.-1 + KN 0.2 mg.lt.-1) exhibit the best response studied. The plant material obtained by this procedure is proposed for pharmacological and chemical studies of this important antimycotic plant remedy.