Top Abstract Purchase Related
Home
>
Node
>
Subnode

RDNA ITS sequence analysis of wild Gentiana from Gansu province

Liu, L.; Wang, X.; Wang, X.; Wang, L.

Zhongguo Zhong Yao Za Zhi 35(5): 565-568

2010

ISSN/ISBN: 1001-5302
PMID: 20506812
Document Number: 643982
To find the patterns of the rDNA ITS sequence variation in Gentiana, and establish the molecular biological method for the identification of the four kinds wild Gentiana from different regions in Gansu. The ITS gene fragments were PCR amplified and sequenced. The rDNA ITS regions were analyzed by means of the software of Clustal X, MEGA3. The Complete ITS sequence of G. macrophylla, G. straminea, G. dahurica and G. officinale was 800 bp. The sequences of ITS1, ITS2 and 5.8S were 290, 340, 170 bp, respectively. Phylogenetic tree based on ITS1 and ITS2 sequences data was constrcuted by Neighbor-joining method. ITS sequence could be as the evidence for the molecular authentication of Gentiana.

Document emailed within 1 workday
Secure & encrypted payments

Yang, R.; Li, D.; Zhao, J. 2001: 26S rDNA D1/D2 domain sequence analysis of the pathogenic strain from the first case of disseminated trichosporonosis in China Zhonghua Yi Xue Za Zhi 81(8): 472-475
Li, H.-Y.; Yu, D.-S.; Chen, J.-H.; Liu, X.-Z. 2008: Analysis of influenza surveillance from 2000 to 2007 in Gansu province Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 22(4): 266-268
Zhang, Z-Long.; Chen, X-Peng.; Yang, J.; Xue, B.; Li, Y-Jin. 2010: Dynamic changes of the relationships between economic growth and environmental pressure in Gansu Province: a structural decomposition analysis Ying Yong Sheng Tai Xue Bao 21(2): 429-433
Diao, Y.; Miao, Y.; Lin, X.; Liao, C.; Guo, F.; Hu, Z. 2009: Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences Genetika 45(4): 506-510
Wang, P.G.; Zhang, X.S.; An, J.; Wang, X.X.; Zhang, N.J.; Tang, Y.; Wang, X.L.; Liang, X.F.; Zhang, B.L.; Jiao, Y.Z.; Bao, J.Y.; Deng, L.; Li, X.B.; Li, H. 2016: Investigation and analysis of the coverage rate of hepatitis B vaccine in Gansu province after 12 years since integrated into expanded programme on immunization Zhonghua Yu Fang Yi Xue Za Zhi 50(6): 541-543
Wang, X.; Liu, X. 2007: Study on the comparison of rDNA and ITS sequence of Fumonisin-producing strains Wei Sheng Yan Jiu 36(1): 55-59
Diao, Y.; Duan, W.-t.; Lin, X.-m.; Liao, C.-l.; Hu, Z.-l.; Wang, Y.-w. 2010: Molecular authentication of Valeriana jatamansi by ARMS based on 5S rDNA sequence Yao Xue Xue Bao 45(8): 1067-1070
Song, D.; Sun, G.-Q.; Zhang, Y.-M.; Cui, W.-G.; Chen, C.-X.; An, H.-J.; An, Y.-H.; Wang, Z.; Bao, Y.-Z. 2012: Subtype and sequence analysis of gag gene of HIV-1 among men who have sex with men in Zhengzhou, Henan Province Bing du Xue Bao 28(4): 345-350
Squire, K.R.; Chuang, R.Y.; Chuang, L.F.; Doi, R.H.; Osburn, B.I. 1986: Sequence relationships of United States prototype and wild-type bluetongue virus RNA genomes investigated by northern blot hybridization analysis American Journal of Veterinary Research 47(1): 53-60
Zhang, W.; Yu, H.; Wang, X.; Wang, Q.; Lu, R.; Wang, H.Q.; Shao, J.J. 2001: Analysis of the nucleotide sequence for C and NS5 regions and the genotype of HCV isolate in Shandong Province Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 15(3): 219-221
Yang, H.; Li, M.; Jiang, Y. 1997: Molecular cloning and sequence analysis of glycoprotein D gene of herpes simplex virus 2 strain Sav and wild strain isolated Zhonghua Yi Xue Za Zhi 77(2): 97-99
Li, J.; Han, P.; Qi, F. 2014: Epidemiological features of metabolic syndrome in Gansu province Zhonghua Liu Xing Bing Xue Za Zhi 35(9): 1015-1019
Yu, C.; Li, S.-J.; Wang, D.-M.; Tang, Q.; Tao, X.-Y.; Li, H.; Zhuang, Y.; Zhou, J.-Z.; Wang, Y.; Tian, K.-C.; Tang, G.-P. 2011: Analysis on nucleoprotein gene sequence of 25 rabies virus isolates in Guizhou Province, China Bing du Xue Bao 27(6): 549-556
Li, J.; Yu, A.; Yang, C.; Wang, B.; Yang, M.; Meng, L. 2014: Situation and correlated behavior of HIV infection among currently married MSM in Gansu province Zhonghua Liu Xing Bing Xue Za Zhi 35(7): 809-811
Marugán de Miguelsanz, J.M.; Lapeña López de Armentia, S.; Rodríguez Fernández, L.M.; Palau Benavides, M.T.; Torres Hinojal, M.C.; Menau Martín, G.; Gutiérrez Fernández, M.; Alvaro Iglesias, E. 1996: An epidemiological analysis of the sequence of bladder control and nocturnal enuresis prevalence in the children of the province of León Anales Espanoles de Pediatria 44(6): 561-567
Lu, J.; Li, H.-y.; Tian, R.-g.; Jiang, Y.-z.; Xu, Y.-n.; Yu, D.-s.; Jiang, J.-x.; Bi, S.-l. 2009: Hepatitis viruses infection situation in human population of the Gansu province Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 23(5): 349-351
Ding, X.L. 1993: Investigations on the epidemiology of brucellosis in some villages (pasturelands) of Su Nan County, Gansu Province Zhonghua Liu Xing Bing Xue Za Zhi 14(6): 338-340
Gou, F.; Liu, X.; Liu, D.; Ren, X.; Li, J.; Liu, H.; Zheng, Y.; Wei, K.; Yang, X.; Cheng, Y.; Meng, L. 2016: Spatial-temporal distribution of hepatitis B in Gansu province, 2009-2014 Zhonghua Liu Xing Bing Xue Za Zhi 37(1): 85-89
Shu, Z.; Yang, Z.-y.; Meng, R.-g.; Zhan, S.-y. 2010: Detection rate on un-repaired cleft lip/palate patients in Gansu province in 2008 Zhonghua Liu Xing Bing Xue Za Zhi 31(6): 659-661
Xu, Q.; Chen, L.-l.; Zhang, R.-h.; Yang, L.; Xie, Z.-j.; Zhu, Y.-l.; Jiang, S.-j.; Si, X.-k. 2013: Sequence analysis of VP1 gene of the duck hepatitis a virus type 3 strains isolated from Shandong Province of China in 2012 Bing du Xue Bao 29(5): 522-528