Analysis of the nucleotide sequence for C and NS5 regions and the genotype of HCV isolate in Shandong Province
Zhang, W.; Yu, H.; Wang, X.; Wang, Q.; Lu, R.; Wang, H.Q.; Shao, J.J.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 15(3): 219-221
2001
ISSN/ISBN: 1003-9279 PMID: 11986688 Document Number: 538050
To study the genotype of HCV epidemic strains in Shandong Province. HCV fragments of C(432 bp) and NS5(319 bp) regions were amplified by RT nested PCR. The fragments were cloned to T vector and sequenced by dideoxynucleotide chain termination reaction. Three of all 4 strains in C region were 1b genotype, and 1 was 2a. All of 10 strains of NS5 region were 1b genotype. No other genotype was found in this region. The homology of nucleotide sequence was over 90% compared with that of more than 50 strains in Gen Bank. The major genotype of HCV epidemic strains in Shandong Province is 1b. The 2a genotype is also found in this area.