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Sequence relationships of United States prototype and wild-type bluetongue virus RNA genomes investigated by northern blot hybridization analysis

Squire, K.R.; Chuang, R.Y.; Chuang, L.F.; Doi, R.H.; Osburn, B.I.

American Journal of Veterinary Research 47(1): 53-60

1986

ISSN/ISBN: 0002-9645
PMID: 3004266
Document Number: 280703
The 10 double-stranded RNA (dsRNA) genome segments of various isolates of bluetongue virus (BTV) were separated on a polyacrylamide gel, denatured in NaOH, and blotted onto 2-aminophenylthioether paper. Blotted dsRNA segments were detected, using radioactive probes, a cloned copy of DNA 70% fragment of genome segment 7 of BTV-17, whole genome BTV-17 copy DNA, or whole genome BTV-17 dsRNA. These probes detected sequence diversities in different isolates of BTV, and these diversities are discussed in relation to the serotype and the electrophoretic migration patterns of the isolates.

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