Assessment of lipid peroxidation level in serum of patients with gastrointestinal tract tumors
Czeczot, H.; Scibior-Bentkowska, D.; Skrzycki, M.Å‚; Podsiad, M.Å‚g.
Wiadomosci Lekarskie 63(3): 180-187
2010
ISSN/ISBN: 0043-5147 PMID: 21125741 Document Number: 643650
Clinical and experimental studies indicate oxygen free radicals and their reactive derivatives participation in formation of chronic inflammation states, which facilitate gastrointestinal tract tumors development. During malignant changes formation in epithelium of gastrointestinal tract increased oxygen radicals generation initiates lipid peroxidation and DNA and proteins oxidation processes. Final lipid peroxidation products (saturated and unsaturated aldehydes) are highly reactive and characterized by greatly longer half life time than reactive oxygen species and capability to diffuse from places of their formation to distant cell areas. In cells they react with important biological macromolecules such as DNA and proteins causing their structural and functional damages. The effects of changes in cell membranes structure are increase in their permeability, depolarization, decrease of hydrophobicity and inhibition of enzymes, membrane channels and transporters. These changes lead to the loss of cell integrity. Determination of lipid peroxidation level in blood serum patients with gastrointestinal tract tumors. Materials for studies were obtained from 170 patients with gastrointestinal tract tumors: 10 with stomach cancer, 20 with pancreatic cancer, 30 with primary liver cancer, 60 with primary colorectal cancer and 50 with metachronic colorectal cancer liver metastases. Blood was taken from patients 1 day before and 6 days after surgery. Control blood was obtained from 53 healthy blood donors. Lipid peroxidation level was determined spectrophotometrically as a concentration of final lipid peroxidation products, which in reaction with tiobarbituric acid (TBA) form colour complex (thiobarbituric acid-reactive substances, TBARS). Higher lipid peroxidation level was observed in pre- and postoperative blood sera patients with gastrointestinal tumors in comparison to serum from healthy blood donors. Increased lipid peroxidation level in peripheral blood of patients with gastrointestinal tract tumors is evidence of intensive oxidative stress and might indicate impairment of antioxidant defense mechanisms in organism.