Comparative analysis of parasite detection methods in vegetables for human consumption
Barnabé, A.S.; Ferraz, R.R.N.; Pincinato, E.d.C.; Gomes, R.C.F.; Galleguillos, T.G.B.; Cerqueira, M.Z.; Soares, E.G.L.; Lage, P.S.; Araújo, C.él.X.; Szamszoryk, M.; Massara, C.L.
Revista Cubana de Medicina Tropical 62(1): 21-27
2010
ISSN/ISBN: 0375-0760 PMID: 23431633 Document Number: 642489
Vegetable contamination is a persistent health problem. The different methods of processing and diagnosis make it difficult to determine the most effective and sensitive technique. a comparative analysis of parasitological technique sensitivity in vegetable samples. a total of 30 samples were harvested -lettuce (Lactuca sativa), rocket (Eruca sativa) and watercress (Nasturtium officinale)--and later analyzed using Hoffman, Pons, and Janer (HPJ) and Faust (f) techniques. Data were analyzed, using the Bland-Altman test to evaluate the correlation and the Mann-Whitney test to compare the medians. of the analyzed samples, 46.6% were positive for intestinal parasites; Balantidium coli, accounting for 20% of contamination, Entamoeba coli (21.6) and Entamoeba histolityca (5%), Trichuris trichiura (3.3%) and Strongyloides stercoralis (2.5%) The Bland-Altman test showed significant correlation between the analyzed methods. When evaluating the averages separately, there was significant difference (p = 0.05) among the results. this study proved that the HPJ technique was more effective for the detection of eggs, helminth larvae and protozoan cysts in the plants under study.