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Comparative analysis of 2 methods of DNA extraction

Sánchez García, I.; Martín Seisdedos, C.; Pérez Grande, R.; Corral de la Calle, J.; Rodríguez Arias, C.; González-Sarmiento, R.

Sangre 35(5): 405-407

1990

ISSN/ISBN: 0036-4355
PMID: 2291150
Document Number: 350871
The study of the organization of immunoglobulin and T cell receptor genes in leukaemias and lymphomas depends on our ability to obtain high molecular weight DNA. We have compared two different methods of DNA extraction: one being enzymatic, using the enzyme proteinase K, and the other chemical, using urea as a substance purifying DNA. Since the amount and purity of the DNA obtained are similar with each of the two methods of DNA extraction, both are valid to make such a genetic analysis.

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