Experiences with HEK293: a Human Cell Line
Rubino, M.J.
PDA Journal of Pharmaceutical Science and Technology 64(5): 392-395
2010
ISSN/ISBN: 1948-2124 PMID: 21502043 Document Number: 640779
The paper describes considerations taken with regard to a recombinant human cell line (HEK293) producing a therapeutic protein. When working with HEK293 cells, besides standard viral safety issues, special precautions should be taken because this cell line is susceptible to infection by numerous viruses. A viral contamination event occurred with this cell line even after a number of precautions were taken.
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