Mechanisms of interaction between HIV and Mycoplasma arginini in vitro
Miller, G.G.; Rakovskaia, I.V.; Pokidysheva, L.N.; Korablina, E.V.; Titova, I.V.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 49-53
2009
ISSN/ISBN: 0372-9311 PMID: 19715203 Document Number: 637566
Until now, the problem of effective therapy of HIV-infection is not resolved due to integrative type of interaction of HIV virus with target cell - T-lymphocyte. The study was aimed on search of method of deletion of HIV DNA-provirus from cell's genome. Non-pathogenic for humans Mycoplasma arginini was used for coinfection of HIV-infected cells in model systems in vitro. Complex of mechanisms was documented leading to: blocking up to 50 - 60% of extracellular virus (according to titration results), cancel of apoptosis in infected cells stained on Hoechst, formation of defective vif(-) virions, which together with arginine-desaminase of M. arginini arrange permissive conditions for activation of cellular APOBEC3G with subsequent disruption of DNA- provirus and blocking of viral infection. As studies of ultrastructure showed, listed events resulted from direct interaction of HIV with mycoplasma. The elimination of HIV DNA-provirus is possible by co-infection of T-lymphocytes with M. arginini.