Influence of cultivation temperature of Yersinia pseudotuberculosis on the immunobiological properties of lipopolysaccharide
Byvalov, A.A.; Eremenko, I.D.; Drobkov, V.I.; Smirnova, I.V.; Gavrilova, L.B.; Pechenkin, D.V.; Kozhemiako, A.V.; Bobrova, L.G.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2: 7-11
2009
ISSN/ISBN: 0372-9311 PMID: 19459469 Document Number: 632202
To study relationship between Yersinia pseudotuberculosis cultivation temperature and immunobiological properties of lypopolysaccharide (LPS). LPS producing strain--Yersinia pseudotuberculosis 164/84 OX; experimental animal--guinea pigs, rabbits; methods--immunochemical (indirect hemagglutionation assay, RDP), physico-chemical (electrophoresis, gas-liquid chromatography), standard biochemical and statistical methods. It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10 degrees C to 37 degrees C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amount of electrophoretically detected O-side chains. It was assumed that the polysaccharide component plays its role in LPS toxicity. Relation between Yersinia pseudotuberculosis cultivation temperature and structural and functional properties of LPS preparations obtained from the culture was determined.