Prevalence of IS285 and IS100 in Yersinia pestis and Yersinia pseudotuberculosis genomes
Bobrov, A.G.; Filippov, A.A.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1997(2): 36-40
1997
ISSN/ISBN: 0208-0613 PMID: 9213772 Document Number: 481639
Cell DNAs of various species of Enterobacteriaceae were hybridized with the probes based on IS285 and IS100, mobile genetic elements of Yersinia pestis. These IS elements are found only in the genomes of all tested Y. pestis strains and a number of strains of a related bacterium Y. pseudotuberculosis. Phylogenetic relations between the tested strains and correlation of fingerprints with the geographical origin of the strains were revealed by analysis of the hybridization profiles of Y. pestis and Y. pseudotuberculosis chromosomal DNAs with IS probes. Comparison of the chromosomal IS100 profiles of Y. pestis wild strain and its nonpigmented mutant helped us determine the minimal extension of the genetic rearrangement and detect at least three copies of the IS element in the mutant region.