Cloning and molecular characteristics analysis of a MYB gene from Fagopyrum dibotrys
Ma, J.; Zhu, Q.; Guo, T.; Liu, G.; Sui, S.; Li, M.
Zhongguo Zhong Yao Za Zhi 34(17): 2155-2159
2009
ISSN/ISBN: 1001-5302 PMID: 19943474 Document Number: 631961
Using RACE with a Fagopyrum dibotrys callus cDNA library, one clone, named FdMYBP1, encoding a putative R2R3 MYB protein was identified. FdMYBP1 appeared to be a full-length cDNA of 1159 bp encoding a protein of 265 amino acids. Through structure and property analysis of FdMYBPI with bioinformational methods, it was found that the amino acid sequence of FdMYBP1 showed great homology to other MYBP with the R2R3 repeat region in the N-terminus. Southern blot analysis indicated that FdMYBP1 belongs to a single copy gene in F. dibotrys genomes. The FdMYBP1 gene has the same classic characters with other MYBP and probably involved in the pathway of flavonoid metabolisms.