Molecular cloning of the imipenem resistant gene in bacteroides fragilis
Zhou, Z.; Fan, X.; Feng, P.; Lü, X.; Lin, J.; Zhang, H.; Lu, Y.
Hua Xi Yi Ke Da Xue Xue Bao 28(2): 184-187
1997
ISSN/ISBN: 0257-7712 PMID: 10683931 Document Number: 480155
Imipenem, as a representative of carbapenem, is one of the most effective beta-Lactam agents against Bacteroides, but recently there have been a few reports on the resistant strains of Bacteroides. To explore the mechanism of imipenem resistance (IMP-r) at the molecular level, we extracted chromosome DNA of the resistant strains and acquired a 1.7 kb fragment of resistant gene of imipenem. Recombinant plasmid was stably reserved in generation after generation in E. coli DH-5. Resistant gene of imipenem was labeled with Dig-11-dUTP by way of random prime, and 40 strains of clinically isolated Bacteroides were detected by the prepared DNA probe. Two IMP-r strains showed hybridization signal, whereas 38 IMP-r strains did not show such a signal. The result suggests that the Dig-labeled IMP-r gene probe can be used in molecular epidemiology for investigating IMP-r strains.