Antigenic properties of LPS extracted from Bacteroides fragilis enterotoxin producing strains
Meisel-Mikołajczyk, F.; Kot, K.; Kłos, W.; Rouyan, G.S.; Mieszała, M.; Gamian, A.
Acta Microbiologica Polonica 48(2): 153-161
1999
ISSN/ISBN: 0137-1320 PMID: 10581671 Document Number: 504089
Antigenic properties of enterotoxigenic B. fragilis (ETBF) strains isolated from humans and pigs in Poland were compared with reference strains. The agglutination and passive haemagglutination, SDS-PAGE analysis and immunoblotting tests as well as analyses of sugars and fatty acids were performed with lipopolysaccharide (LPS) preparations obtained from water-phase of phenol-water extracts. Some differences in serological reactivity between ETBF antigens were observed. The antigen of the NTBF (non-enterotoxigenic) reference strain IPLE-323 expressed weak cross-reactivity with sera against whole cells of ETBF strains in serological tests. There were some differences observed between ETBF and NTBF strains in fatty acids and sugar composition. It is concluded that the LPS preparations probably possess a common core structure and the O-specific polysaccharides of variable chain length.