Differential effect of phenothiazines on MRP1 and P-glycoprotein activity
Wesołowska, O.; Molnar, J.; Ocsovszki, I.; Michalak, K.
In Vivo 23(6): 943-947
2009
ISSN/ISBN: 0258-851X PMID: 20023237 Document Number: 631038
Overexpression of ATP-binding cassette (ABC) transporters such as P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1) or breast cancer resistance protein (BCRP) accounts for majority of cases of multidrug resistance (MDR) of cancer cells. In the present work, the interactions of seven commercially available phenothiazine derivatives, known P-glycoprotein inhibitors, with this transporter and MRP1 were compared. By flow cytometry, it was shown that all the drugs increased the accumulation of rhodamine 123 in the P-gp-overexpressing lymphoma cell line L5178 MDR. On the other hand, phenothiazine derivatives stimulated MRP1-mediated efflux of fluorescent probe (BCPCF) out of human erythrocytes. In this way, these phenothiazine derivatives were identified as a group of atypical MDR modulators that differently interact with P-gp (as inhibitors) and MRP1 (as stimulators). This observation clearly shows that the activity of all new modulators should be tested for their effects towards different ABC transporters as a standard procedure.