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Thermodynamic characterization of di-, tri-, and tetranucleotide loci in parthenogenetic lizards Darevskia unisexualis

Omelchenko, A.V.; Korchagin, V.I.

Genetika 45(8): 1143-1147

2009

ISSN/ISBN: 0016-6758
PMID: 19769305
Document Number: 630761
Allelic polymorphism of three microsatellite loci from the genome of parthenogenetic lizard Darevskia unisexualis was characterized using analysis of free energy (Gibbs energy) of the DNA/DNA duplex formation within the stepwise mutational model. It was demonstrated that the number of microsatellite cluster monomericic units would change to decrease the mean free energy of the locus. In addition, based on the analysis of nucleotide composition, the GC content of each locus was evaluated, and belonging of the loci examined to certain isochore families was suggested.

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