Primry research of separation and culture of adult hepatocytes

Liu, H.-l.; Xin, S.-j.; You, S.-l.; Hu, Y.; Rong, Y.-h.; Zhou, L.; Yang, Y.-p.; Li, Z.-w.; Liu, Z.-w.; Mao, P.-y.; Liu, H.-l; Xin, S.-j; You, S.-l; Hu, Y; Rong, Y.-h; Zhou, L; Yang, Y.-p; Li, Z.-w; Liu, Z.-w; Mao, P.-y

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Primry research of separation and culture of adult hepatocytes

Liu, H.-l.; Xin, S.-j.; You, S.-l.; Hu, Y.; Rong, Y.-h.; Zhou, L.; Yang, Y.-p.; Li, Z.-w.; Liu, Z.-w.; Mao, P.-y.

Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 22(5): 376-378

2008

ISSN/ISBN: 1003-9279

PMID: 19469180

Document Number: 617078

To explore the separation and culture method of adult hepatocytes. The isolated adult hepatocytes were cultivated by RPMI 1640 medium at 37 degrees C in vitro. The characteristics of the growing hepatocytes were observed. Their synthesis of urea was detected. The transformation efficiency and density's change of lidocaine were analyzed. Hepatocytes were successful separated from adult liver. And they were cultivated in common condition and hollow fiber reactor. The functional capacity of hepatocytes was for lidocaine metabolism and urea excretion. The adult hepatocytes have been successful separated from liver. And they can be cultivated in common condition and hollow fiber reactor. And it could provide a great quantity and high activity of hepatocytes for bioartificial liver.