Top Abstract Purchase Related
Home
>
Node
>
Subnode

Primary culture of hepatocytes of newborn rats as a system for modeling anoxia and metabolic ischemia

Goncharenko, T.M.; Nikulina, S.E.; Krylova, O.I.; Stvolinskaia, N.S.; Poliakova, E.D.; Korovkin, B.F.

Voprosy Meditsinskoi Khimii 35(6): 51-55

1989

ISSN/ISBN: 0042-8809
PMID: 2560870
Document Number: 335449
A primary culture of hepatocytes from new born rats was used as an experimental model suitable for studies of the cell lysosomal apparatus and of molecular mechanisms involving cyclic nucleotides for initiation of lysosomal response to extremal conditions. Substrate deficiency and anoxia of the cultivated hepatocytes were found to cause distinct alterations in the state of lysosomal apparatus accompanied by an increase in free activity of acid phosphatase.

Document emailed within 1 workday
Secure & encrypted payments

Stvolinskaia, N.S.; Goncharenko, T.M.; Isakin, V.P.; Dolotov, V.I.; Komm, S.G.; Korovkin, B.F. 1990: The morphological changes in a primary culture of 3-day-old rat hepatocytes cultured over 1 week and in a model of anoxia Tsitologiia 32(9): 863-867
Krumschnabel, G.; Schwarzbaum, P.J.; Biasi, C.; Dorigatti, M.; Wieser, W. 1997: Effects of energy limitation on Ca2+ and K+ homeostasis in anoxia-tolerant and anoxia-intolerant hepatocytes American Journal of Physiology 273(1 Pt 2): R307-R316
Land, S.C.; Buck, L.T.; Hochachka, P.W. 1993: Response of protein synthesis to anoxia and recovery in anoxia-tolerant hepatocytes American Journal of Physiology 265(1 Pt 2): R41-R48
Sawada, N.; Furukawa, K.; Gotoh, M.; Mochizuki, Y.; Tsukada, H. 1982: Primary culture of preneoplastic hepatocytes from rats treated with 2-acetylaminofluorene and clofibrate: relationship between resistance to dimethylnitrosamine and responsiveness of peroxisomes Gan 73(1): 1-6
Miyazaki, M.; Namba, M. 1992: Cell culture and its application primary culture of human hepatocytes and its application Gan to Kagaku Ryoho. Cancer and ChemoTherapy 19(9): 1411-1419
Wegner, H.; Schareck, W.; Bayer-Helms, H.; Gebhardt, R. 1992: Different proliferative potential of rat and pig hepatocytes in pure primary culture and coculture European Journal of Cell Biology 58(2): 411-417
Guzelian, P.S.; Bissell, D.M.; Meyer, U.A. 1977: Drug metabolism in adult rat hepatocytes in primary monolayer culture Gastroenterology 72(6): 1232-1239
Wiebkin, P.; Dees, J.H.; Mathis, J.M.; Prough, R.A. 1985: Drug metabolism by isolated fetal human hepatocytes in suspension and primary culture Drug Metabolism and Disposition: the Biological Fate of Chemicals 13(2): 163-168
Schwarz, K.R.; Lanier, S.M.; Carter, E.A.; Homcy, C.J.; Graham, R.M. 1985: Rapid reciprocal changes in adrenergic receptors in intact isolated hepatocytes during primary cell culture Molecular Pharmacology 27(2): 200-209
Mori, H.; Kawai, K.; Ohbayashi, F.; Kuniyasu, T.; Yamazaki, M.; Hamasaki, T.; Williams, G.M. 1984: Genotoxicity of a variety of mycotoxins in the hepatocyte primary culture/DNA repair test using rat and mouse hepatocytes Cancer Research 44(7): 2918-2923
Sun, E.L.; Feenstra, K.L.; Bell, F.P.; Sanders, P.E.; Slatter, J.G.; Ulrich, R.G. 1996: Biotransformation of lifibrol (U-83860) to mixed glyceride metabolites by rat and human hepatocytes in primary culture Drug Metabolism and Disposition: the Biological Fate of Chemicals 24(2): 221-231
Flendrig, L.M.; Maas, M.A.; Daalhuisen, J.; Ladiges, N.C.; La Soe, J.W.; Te Velde, A.A.; Chamuleau, R.A. 1998: Does the extend of the culture time of primary hepatocytes in a bioreactor affect the treatment efficacy of a bioartificial liver? International Journal of Artificial Organs 21(9): 542-547
Abdel-Razzak, Z.; Loyer, P.; Fautrel, A.; Gautier, J.C.; Corcos, L.; Turlin, B.; Beaune, P.; Guillouzo, A. 1993: Cytokines down-regulate expression of major cytochrome P-450 enzymes in adult human hepatocytes in primary culture Molecular Pharmacology 44(4): 707-715
Kayano, T.; Nomura, K.; Hino, O.; Kitagawa, T. 1982: Failure of phorbol ester TPA to promote growth of in vivo-initiated rat hepatocytes transferred into a culture system Gan 73(3): 354-357
Grek, O.R.; Sharapov, V.I.; Sokirchenko, I.A.; Shkurupiĭ, V.A. 1987: Prevention, using alpha-tocopherol and lidocaine, of damage to the monooxygenase system activity and ultrastructure of hepatocytes following acute ischemia of the liver Biulleten' Eksperimental'noi Biologii i Meditsiny 104(12): 669-671
Dauranov, I.G. 1974: Some metabolic changes in the lymphocytes of newborn rats during asphyxia Biulleten' Eksperimental'noi Biologii i Meditsiny 78(12): 21-23
Pylypko, I.; Halytska-Kharkhalis, O.; Henyk, T.; Flekey, N.; Panchyshyn, N. 2018: Morphological Changes of the Urogenital System Internal Structure at Modeling of Portal Hypertension in Rats Georgian Medical News 2018(279): 117-122
Santhosh, A.; Nair, K.G.; Arun, P.; Deepadevi, K.V.; Manojkumar, V.; Lakshmi, L.R.; Kurup, P.A. 1998: Effect of DEHP [di-(2-ethyl hexyl) phthalate] on lipid peroxidation in liver in rats and in primary cultures of rat hepatocytes Indian Journal of Medical Research 108: 17-23
Kao, R.; Rannels, D.E.; Morgan, H.E. 1976: Effects of anoxia and ischemia on protein synthesis in perfused rat hearts Circulation Research 38(5 Suppl 1: I124-I130
Van Belle, H.; Goossens, F.; Wynants, J. 1987: Formation and release of purine catabolites during hypoperfusion, anoxia, and ischemia American Journal of Physiology 252(5 Pt 2): H886-H893