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The morphological changes in a primary culture of 3-day-old rat hepatocytes cultured over 1 week and in a model of anoxia

Stvolinskaia, N.S.; Goncharenko, T.M.; Isakin, V.P.; Dolotov, V.I.; Komm, S.G.; Korovkin, B.F.

Tsitologiia 32(9): 863-867

1990

ISSN/ISBN: 0041-3771
PMID: 2291240
Document Number: 352617
A direct observation of primary cultures of 3-day-old rat hepatocytes was performed with the help of time-consuming cinematography. The process of monolayer formation has been examined. In addition, the continuous preservation of bile canaliculi was observed. A significant increase in hepatocyte mobility was marked in 6-7-day cultures. The structural analysis of cultured cells during the modelling of anoxia with substrate deficiency revealed a succession in hepatocyte and fibroblast alterations. The hepatocyte destruction was accompanied with changes in the cell form, that become rounded, and in the appearance of small formations protruding on the cell surface. The fibroblast destruction appeared after cell contracture.

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