Detection of Adoxophyes orana granulovirus in Adoxophyes orana by PCR
Kundu, J.K.; Zichová, T.; Stará, J.; Kocourek, F.
Acta Virologica 52(3): 187-188
2008
ISSN/ISBN: 0001-723X PMID: 18999895 Document Number: 615195
This paper describes a PCR assay that has proved to be specific for the detection of AdorGV in Adoxophyes orana. The primer pair AdorGraF/AdorGraR specifically amplifies a 620-bp fragment of granulin gene of AdorGV. Using this assay, we detected AdorGV in larvae of A. orana from the virus-treated orchards, Libeany and Slan. Furthermore, AdorGV was not detected in the larvae of A. orana from natural population not treated with virus.
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