Novel detection system of flow injection analysis (1) . the existence of significant relation between secondary structure of DNA and sensitivity in signal detection
Sawata, S.; Kai, E.; Ikebukuro, K.; Iida, T.; Honda, T.; Karube, I.
Nucleic Acids Symposium Series 37: 247-248
1997
ISSN/ISBN: 0261-3166 PMID: 9586092 Document Number: 469861
Polymerase Chain Reaction (PCR) products was detected quantitatively using a flow injection type sensor, based on Surface Plasmon Resonance (SPR). We used asymmetric PCR to amplify the two kinds of products; their DNA lengths are different. This novel design permitted us not only to detect PCR products with high-sensitivity, but also to develop a rapid DNA detection system for the sense of the genetic pathogen.