Expression and biological activities of arresten in CHO cells
Long, M-yun.; Zheng, Q-chang.; Song, Z-fang.; Hu, Q-gang.; Zhang, Y.
Zhonghua Zhong Liu Za Zhi 29(4): 249-252
2007
ISSN/ISBN: 0253-3766 PMID: 17760248 Document Number: 612863
Objective To explore the eukaryotic expression of arresten in CHO cells and to investigate its basic biological activities. Methods CHO cells were divided into three groups: transfected pSecTag-arresten group, transfected pSecTag group and control group without transfection. PSecTag-arresten was transfected into CHO cells by Lipofectamine 2000 method. The arresten mRNA in CHO cells was assayed by RT-PCR. The protein expression of arresten gene was examined by Western-Blot. The cells expressing arresten were screened out by Zeocin. The effect of arresten on huvec cell migration and anchoring to three-dimensional vascular structures was measured. Results The result of RT-PCR and Western-blot showed that arresten gene has been successfully transfected into CHO cells and expressed in those cells. Arrssten inhibited huvec cell migration and anchoring to three-dimensional vascular structures. Conclusion CHO cells expressing arresten have been obtained successfully. Arresten can inhibit huvec cell migration and anchoring to three-dimensional vascular structures, indicating that it might be one of its anti-angiogenetic approaches.