Chromatographic fingerprint of Cnidium monnieri
Yang, X.; Yang, S-Ping.; Zhang, X.
Yao Xue Xue Bao 42(8): 877-881
2007
ISSN/ISBN: 0513-4870 PMID: 17944239 Document Number: 611740
To establish the method of HPLC-fingerprint analysis for the quality control of Cnidium monnieri L. Cuss. and identify its active constituents by HPLC-MS, 35 batches of samples were analyzed on a Shimadzu C-18 column with a gradient of acetonitrile and 0.1 % aqueous aceticacid at a flow rate of 1.0 mL.min(-1) and detected at 245 nm and 322 nm. Furthermore, the typical samples were detected by HPLC-DAD-MS under negative ion mode. 35 batches of Cnidium monnieri L. Cuss. samples were classified into four types based on the results of similarity analysis. According to the comparison of the t(R), MS data and UV maximum absorbance (lambda(max)) values with the standards, 8, 7, 4 and 2 coumarins components were identified in four types of Cnidium monnieri L. Cuss. extracts, separately. The method is repeatable and reliable, and it is capable of effectively controlling the quality of Cnidlum monnieri L. Cuss.