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Cloning NS1 gene of H5N1 avian influenza virus and apoptosis induced by it in human pulmonary carcinoma cell line A549

Zhang, C.-F.; Jiang, S.-W.; Zhu, H.-Q.; Yang, Y.-T.; Yang, Z.-X.; Xu, L.; Zhao, L.-X.; Zhou, X.-W.; Huang, P.-T.

Bing du Xue Bao 23(5): 360-365

2007

ISSN/ISBN: 1000-8721
PMID: 17969852
Document Number: 607180
The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR, and the am-plified product was cloned into the eukaryotic expression vector pCMV-Myc, then it was transfected into A549 cells. After 48 h, the expression of NS1 was detected by Western blot. Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apop-tosis in human pulmonary carcinoma cell line A549.

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