Formation of Pseudorabies virus glycoprotein E/I complex in baculovirus recombinant system
Tyborowska, J.; Reszka, N.; Kochan, G.; Szewczyk, B.
Acta Virologica 50(3): 169-174
2006
ISSN/ISBN: 0001-723X PMID: 17131935 Document Number: 603218
Glycoproteins E (gE) and I (gI) of Pseudorabies virus (PRV) form a non-covalently bound complex to which a number of functions have been attributed. The gE/gI complex formation was studied using a series of full-length and truncated forms of gE and gI expressed in baculovirus recombinant system. Both glycoproteins were truncated by stepwise removal of their C-terminal parts and their ability to form the complex was Studied by radioimmunoprecipitation. It was found that N-terminal domains of gE and gI containing first 122 and 106 aa, respectively, were sufficient for the complex formation.
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