Identification of the components and products of hydrolysis in acetylleucomycin by LC-MS
Hu, M.; Hu, C-Qin.
Yao Xue Xue Bao 41(5): 476-480
2006
ISSN/ISBN: 0513-4870 PMID: 16848328 Document Number: 603019
Aim To identify the components of acetylleucomycin and its hydrolytic products by LG MS. Methods acetylleueomycin was separated on a Diamonsil C,, column with 0.1 mol (.) L-1 ammonium aectate-aeetontrile ( 35: 65) as mobile phase. The LC-MS was equipped with an electorspray ion source (ESI), which was set at the positive ion mode, and the mass spectra of each component in chromatogram were obtained with difference cone voltage. Results The components of acetylleucomycin and its hydrolytic products can be separated by HPLC. The components were identified according to the molecular weight and its major mass fragment ions. The major components identified In domastic acetylleucomycin were acetylleucomycin A(4), A(5); acetylleucomycin A(1), A(3); aeetylleucomycin A(6), A(7), and acetylleucomyein A(13). The hydrolytic products of acetylleucomycin were not kitasamycin, but some non-complete hydrolytic product. Conclusion The method is rapid, sensitive and specific. It's suitable to application in the fields of multi-components antibiotics analysis.