Estimation of ethanol concentrations in biological media. Comparison of alkylnitrite and direct vapor-phase methods
Barinskaia, T.O.; Tiurin, I.A.; Smirnov, A.V.
Sudebno-Meditsinskaia Ekspertiza 49(6): 32-34
2006
ISSN/ISBN: 0039-4521 PMID: 17191726 Document Number: 595630
Ethanol assays in the blood, saliva and urine were made according to two methods: alkylnitrite gas-liquid chromatography and direct vapor-phase method without thermostating. N-propanol was used as an internal standard. For saliva and urine the methods produced similar results. For blood, the direct vapor-phase method detected significantly lower level of ethanol compared to alkylnitrite one (p = 0.001). 24-h storage of the samples in refrigerator does not deviate the results. Recommendations are provided for most effective usage of each method.