Comparison of Four Direct Homogeneous Methods for the Measurement of Low-Density Lipoprotein Cholesterol
Yamada, K.; Tsuji, N.; Fujita, T.; Tanaka, M.; Kuribasyashi, K.; Kobayashi, D.; Watanabe, N.
Clinical Laboratory 56(7-8): 327-333
2010
ISSN/ISBN: 1433-6510 PMID: 20857897 Document Number: 11684
Background: The primary lipoprotein risk factor is low-density lipoprotein cholesterol (LDL-C), and medication is targeted at lowering LDL-C values. To clarify the usefulness of direct homogeneous assays for LDL-C measurement, we compared the values obtained by various reagents to those obtained by the Friedewald equation and analyzed different reactivity to IDL/VLDL and LDL. Methods: Serum samples were collected from 55 patients with hypercholesterolemia. The LDL-C concentrations were determined by four direct homogeneous assays using reagent A (Kyowa Medex), B (Sekisui Medical), C (Denka Seiken), and D (Sysmex), which are commercially available. Results: Significant correlation was observed in LDL-C values obtained by the homogeneous assays and the Friedewald equation. However, there were two discrepancies in reagents B and C, respectively. These assays showed 40 % and 55 % lower LDL-C values than those calculated by the Friedewald equation, respectively. Reactivity to the IDL fraction in reagents B and C was lower than in reagents A and D. Conclusions: Direct homogeneous assays for LDL-C are suitable for routine laboratory examination. However, it was shown that attention should be given to the different reactivity to IDL and LDL among reagents in some clinical samples.
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