Comparison of High-Sensitivity C-Reactive Protein Serum Assay Results Obtained Using Dade-Behring BNII Nephelometer and Ortho Vitros FS 5.1 Clinical Analyzer in Respect of CRP-Related Risk Assessment of Chronic Metabolic Diseases
Beata Kusnierz-Cabala; Gernand, W.; Zabek, A.-Adamska; Tokarz, A.; Naskalski, J.W.
Clinical Laboratory 54(9-10): 341-346
2008
ISSN/ISBN: 1433-6510 PMID: 19097491 Document Number: 11685
Background: Serum concentration of high sensitive C-reactive protein (hsCRP) can predict the risk of chronic metabolic and cardiovascular diseases but it is unclear whether turbidimetric high sensitive assays of CRP are adequate. Methods: Concentrations of serum CRP in 126 samples of serum were measured with high-sensitivity methods using nephelometry (BN II Nephelometer) and turbidimetry (Ortho Vitros FS 5.1). Results: For CRP concentrations measured by nephelometry and turbidimetry intra-assay CVs were 3.2 and 0.9% at mean CRP concentrations of 1.4 and 2.1 mg/l, inter-assay CVs for commercial controls were 3.1% and 3.6% at mean concentrations of 1.3 and 1.7 mg/l, and mean biases were 7.62% and 2.26%, respectively. Measurements were strongly, linearly correlated (r=0.99; CRP Vitros =0.03+1.03CRP BNII ). When disease risk was assessed by nephelometry and turbidimetry, results were similar. If the risk of disease was classified as moderate (1.0 3.0 mg/l), the frequency of misclassified cases was only 2.3 and 2.1%, respectively. The classification agreement weighted ĸ coefficient was 0.94 (95% C.I.: 0.89-0.98). Conclusions: turbidimetric high sensitive CRP assays can properly classify CRP-related prediction of chronic metabolic diseases with special consideration on cardiovascular risk.
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