Comparison of plasmid DNA preparation methods for direct gene transfer and genetic immunization
Davis, H.L.; Schleef, M.; Moritz, P.; Mancini, M.; Schorr, J.; Whalen, R.G.
Biotechniques 21(1): 92
1996
ISSN/ISBN: 0736-6205 PMID: 8816242 Document Number: 468992
Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.
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