Comparison of Diagnostic Effectiveness of Methods of Detection of Coxiella Burnetii in Blood of Patients with Q Fever Based on Amplifi- Cation of 16s Rrna Gene Fragments (Standard Pcr) and Groel Gene (Real- time Pcr)
Panferova, Y.A.; Freilikhman, O.A.; Tokarevich, N.K.; Karpenko, S.F.; Galimzyanov, K.M.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 3: 70-74
2016
ISSN/ISBN: 0372-9311 PMID: 30695456 Document Number: 687625
Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burneli persistence in dynamics of infectious process in patients with Q fever. 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time. PCR (RT-PCR) (marker - groEL gene fragment). Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31). This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process.