Validation of an internal method for the diagnosis of infections with Chlamydophila abortus and Coxiella burnetii by real-time multiplex PCR
Pelletier, C.; Chartier, S.; Berthillier, J.; Dégletagne, E.; Rigaud, C.; Berthet, H.; Valognes, A.; Reynaud, A.; Véry, P.
Developments in Biologicals 126: 219-26; Discussion 326-7
2006
ISSN/ISBN: 1424-6074 PMID: 17058498 Document Number: 596535
Chlamydophila abortus and Coxiella burnetii are one of the major pathogens implicated in abortion in cattle. Their characteristic of obligate intracellular bacteria, and of zoonotic agents, makes their culture difficult in diagnostic laboratories, and the traditional tools of diagnosis (detection of sera antibodies by ELISA, Stamp's coloration) encounter specificity, sensitivity and interpretability limits. Individual PCR have recently been developed. Nevertheless, their income/cost is a limiting factor for breeders. As the symptoms are not specific, the request for analysis often concerns the two valences. Consequently, the development and the validation of an internal multiplex PCR appears to be a suitable solution.