Topographic labeling method using 3H-proline in assessment of skeletal growth and remodeling in 5-week-old mice
Tonna, E.A.
Laboratory Investigation; a Journal of Technical Methods and Pathology 30(2): 161-169
1974
ISSN/ISBN: 0023-6837 PMID: 4817819 Document Number: 5927
The present report represents an extension of initial 311-proline autoradiographic studies designed to provide, at both the cytologic and histologic levels, an indelible topographic record of skeletal events in 5-week-old mice. In addition, a comparison is made between this method and other methods used as bone markers. 3H-Proline was administered in single and multiple doses and the animals were serially killed between 4 hours and 14 days. Femoral samples sectioned in different planes were prepared for autoradiography and subsequent micrometric and grain count analysis of the resulting silver topogragraphic bands. These bands, coincidental with the cellular uptake and turnover of radiotracer, allowed for the assessment of cellular rates of labeled matrical precursor production and deposition from which the processes of skeletal growth and remodeling can be evaluated. In addition, composite tracings of topographic data derived from multiple sections made at different geometric planes allowed for threedimensional mapping of skeletal events. It was concluded from the present study that the 311-proline topographic method is a sensitive technique offering greater refinement and broader application than presently used fluorochromes and tetracyclines, adding a new dimension to the study and solution of current skeletodental problems. Above all, important quantitative cellular information is provided in conjunction with matrical information.
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