PCR-RFLP for Campylobacter jejuni subtyping
Giacoboni, G.; Echeverría, M.G.; Perfumo, C.
Revista Argentina de Microbiologia 37(2): 81-83
2005
ISSN/ISBN: 0325-7541 PMID: 16178461 Document Number: 592236
Ten Campylobacter jejuni isolates, eight identified as C. jejuni biotype II of Lior and two as C. jejuni biotype I, were recovered from aborted pig fetuses. In order to discriminate among strains, restriction fragment length polymorphism (RFLP) using DdeI of polymerase chain reaction (PCR) products of flaA gene was used. C. jejuni biotype II strains could be differentiated in six strains by PCR-RFLP, and one subtype was obtained from C. jejuni biotype I. Although there is great variability of molecular techniques applied to the Campylobacter epidemiological studies, PCR-RFLP demonstrated to be a simple and accessible technique to discriminate Campylobacter jejuni isolates.