Application of HMGN2-tag constructs to analysis of HMGN2 distribution in Hela cells
Xiong, W.; Feng, Y.; Huang, N.; Wu, Q.; Li, X.; Wang, B.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 22(5): 1015-1019
2005
ISSN/ISBN: 1001-5515 PMID: 16294743 Document Number: 592204
This study sought to clarify the distribution of HMGN2 in HeLa cells. The recombinant eukaryotic expression vectors pcDNA3. 1-myc-his-HMGN2 and pEGFP-N1-HMGN2 were constructed, and then were transfected into HeLa cells. immunocytochemistry staining indicated that HMGN2 were present not only in HeLa nucleus but also in the cytoplasm. The presence of HMGN2 was also detected in the culture supernatant by ELISA with rabbit anti-serum against HMGN2 and mouse anti-His6 monoclonal antibodies. The confocal microscope observation showed the same subcellular localization as that of immunocytochemistry staining. There results suggested that HMGN2 could be present in the nucleus and cytoplasm of HeLa cell as well as in the extracellular environment.