Flow cytometric analysis of pentakis (aziridino) thiatriazadiphosphorine oxide (SOAz) -induced changes in cell cycle progression of Hela and HL-60 cells
Hecquet, C.; Nafziger, J.; Ronot, X.; Marie, J.P.; Adolphe, M.
Cancer Research 45(2): 552-554
1985
ISSN/ISBN: 0008-5472 PMID: 3855380 Document Number: 249662
The treatment of HeLa and HL-60 cells with various concentrations of pentakis(arizidino)thiatriazadiphosphorine oxide results in inhibition of growth and modification of cell cycle distribution. These phenomena were observed at 10(-4) M and 5 X 10(-5) M for HeLa cells and 10(-5) M and 5 X 10(-6) M for HL-60 cells. The estimation of DNA content by flow cytometry showed an important shift in the distribution of cycling cells with a striking arrest in G2 for both cell lines with a concomitant late S-phase accumulation for HeLa cells. Incubation of cells in drug-free medium 3 days after treatment did not show any change in DNA distribution, suggesting the irreversibility of drug action.