Effect of sulphamethazine on the gene expression of FRTL-5 cells
Wang, J.-T.; Sun, D.; Pan, H.-M.; Wu, D.-S.
Wei Sheng Yan Jiu 34(1): 19-21
2005
ISSN/ISBN: 1000-8020 PMID: 15862012 Document Number: 591260
To study the effect of Sulphamethazine on the gene expression of FRTL-5 cells, and to explore the mechaniams of environmental thyroid hormone disruptors. cDNA microarray technique was used to analyze the gene expression of FRTL-5 cells of exponential phase treated by 2.0 microg/ml Sulphamethazine for 24 h. Total RNA from treated and untreated cells were labeled by Cy3 dCTP and Cy5 dCTP respectively. The ratios of Cy3/Cy5 were calculated in order to find the genes which expressed differently. There were 679 genes (approximately 7%, total 9753 genes) exhibiting different expression, in which 395 (4.0%) genes up-regulated and 284 (3.0%) genes down-regulated. These genes related to regulation of gene expression, regulation of cell cycle, metabolism in cells, and so on. The effect of Sulphamethazine on FRTL-5 cells may be related with a series of genes.