Studies on the anticancer effects of total alkaloid from Viscum coloratum
Peng, H-yan.; Zhang, Y-hong.; Han, Y.; Wang, M.
Zhongguo Zhong Yao Za Zhi 30(5): 381
2005
ISSN/ISBN: 1001-5302 PMID: 15806976 Document Number: 587189
Objective: To study the anticancer effects of total alkaloid from Viscum coloratum in vivo and vitro. Method: In vitro, MTT assays were used to measure the inhibitory effect. Cells at period of logarithmic growth were incubated for 24 hours. Then total alkaloid of various concentrations were added. 24 hours later, supernatant was removed and WIT was added. 4 hours after that, DMSO was added, then 30 minutes later, A value was measured. In vivo, suspension of carcinoma cells was implanted in the mice's limbs subcutaneously, 0.2 mL each. 24 hours later, the mice were grouped randomly. Fed by total alkaloid continuously for 7 days, the mice were sacrificed. The tumors were weighed and calculated the inhibitory rates. Result: In vitro, it shows that total alkaloid has prominent inhibitory effect on the growth of carcinoma cells. In vivo, it shows that total alkaloid can inhibit the growth of tumors and prolong the survival days of the mice bearing tumors. Conclusion: Total alkaloid from Viscum. coloratum has the activities of anticancer.