Quaternary structure of NAD-kinase from rabbit liver
Nguyen, V.T.; Telepneva, V.I.
Biokhimiia 46(3): 435-443
1981
ISSN/ISBN: 0320-9725 PMID: 6263371 Document Number: 5691
A procedure for isolation of NAD-kinase from rabbit liver resulting in 4000-fold purification and the activity yield of 50-60% is described. The molecular weight of the NAD-kinase subunit determined by SDS electrophoresis is 30 000. The purified enzyme is a dimer. Partially purified preparations of NAD kinase contain multiple forms with mol. Weights ranging from 650 000 to 180 000 and have complex kinetic behaviour. A thermostable activator of NAD-kinase which, when added to the homogeneous enzyme preparation, destroys the linear dependence of the enzyme specific activity on concentration, was detected. The nature of multiple forms of NAD-kinase and the possible role of the activator in their formation is discussed.
Document emailed within 1 workday
