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Amino acid composition and quaternary structure of glucosoisomerase (d-xylose-ketol-isomerase) from Actinomyces olivocinereus 154

Rezchikov, A.A.; Ulezlo, I.V.; Ananichev, A.V.; Bezborodov, A.M.

Prikladnaia Biokhimiia i Mikrobiologiia 16(4): 547-553

1980

ISSN/ISBN: 0555-1099
PMID: 7220510
Document Number: 158879
The amino acid composition of glucosoisomerase from Actinomyces olivocinereus 154 was investigated. The content of dicarboxylic acids--aspartic and glutamic--was found to be greater than that of basic acids--lysine, arginine and histidine. Hydrophobic acids were also detected to occur on appreciable quantities. No cysteine was seen in the enzyme. The experimental data on the effect of sodium dodecyl sulfate and urea suggests that the enzyme has a quaternary structure consisting of four nonidentical subunits.

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