1- (5-Isoquinolinesulfonyl) -2-methylpiperazine (H-7) is a selective inhibitor of protein kinase C in rabbit platelets
Kawamoto, S.; Hidaka, H.
Biochemical and Biophysical Research Communications 125(1): 258-264
1984
ISSN/ISBN: 0006-291X PMID: 6239622 Document Number: 231994
Effects of 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), a potent inhibitor of protein kinase C in vitro, were investigated with regard to stimulus-induced protein phosphorylation of rabbit platelets. While H-7 inhibited the protein kinase C-mediated phosphorylation in 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated platelets, this compound did not block the Ca2+-calmodulin-dependent phosphorylation in Ca2+ ionophore A23187-stimulated cells. This selective inhibitor of protein kinase C, in intact cells, will facilitate studies on the biological functions of protein kinase C.