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Protein-tyrosine kinase p72syk is activated by platelet activating factor in platelets

Rezaul, K.; Yanagi, S.; Sada, K.; Taniguchi, T.; Yamamura, H.

Thrombosis and Haemostasis 72(6): 937-941

1994

ISSN/ISBN: 0340-6245
PMID: 7740467
Document Number: 434284
It has been demonstrated that activation of platelets by platelet-activating factor (PAF) results in a dramatic increase in tyrosine phosphorylation of several cellular proteins. We report here that p72-syk is a potential candidate for the protein-tyrosine phosphorylation following PAF stimulation in porcine platelets. Immunoprecipitation kinase assay revealed that PAF stimulation resulted in a rapid activation of p72-syk which peaked at 10 s. The level of activation was found to be dose dependent and could be completely inhibited by the PAF receptor antagonist, CV3988. Phosphorylation at the tyrosine residues of p72-syk coincided with activation of p72-syk. Pretreatment of platelets with aspirin and apyrase did not affect PAF induced activation of p72-syk. Furthermore, genistein, a potent protein-tyrosine-kinase inhibitor, diminished PAF-induced p72-syk activation and Ca-2+ mobilization as well as platelet aggregation. These results suggest that p72-syk may play a critical role in PAF-induced aggregation, possibly through regulation of Ca-2+ mobilization.

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