Biosynthetic labelling of platelet activating factor from radioactive acetate by stimulated platelets
Chap, H.; Mauco, G.; Simon, M.F.; Benveniste, J.; Douste-Blazy, L.
Nature 289(5795): 312-314
1981
ISSN/ISBN: 0028-0836 PMID: 7453828 Document Number: 180400
Platelet activating factor (PAF-acether) is a chemical mediator released by (rabbit) basophils in in vitro and in vivo conditions of immunological challenge. PAF-acether induces aggregation and secretion of platelets. Based on several lines of evidence, 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (1-alkyl-2-acetyl-GPC) was recently proposed as the chemical structure of the mediator. As PAF-acether synthesis occurs rather early in activated platelets, one of the most efficient pathways of PAF-acether formation could involve a rapid acetylation of the corresponding lysoderivative (1-alkyl-GPC). Platelets activated by ionophore A23187 are able to incorporate acetate into a mixture of 1-alkyl-2-acetyl-GPC and 1-acyl-2-acetyl-GPC; the former supports the biological activity of PAF-acether.