Protective effects of shenmai injection on DPPH free radical-induced injuries in isolated hearts in rabbits
Li, N-sheng.; Xiong, X-ming.; Liu, L-ying.
Hunan Yi Ke Da Xue Xue Bao 28(3): 247-250
2003
ISSN/ISBN: 1000-5625 PMID: 14653080 Document Number: 562730
Objective: To examine the protective effects of shenmai injection (SM) on DPPH free radical-induced myocardial injury in isoiated hearts in rabbits. Methods: The isolated rabbit hearts were perfused in a Langendorff model. The left ventricular pressure (LVP), the first derivative of LVP (+dp/dtmax), left ventricular end-diastolic pressure (LVEDP) and heart rate (HR) were recorded. Coronary flow (CF) and creatine Kinase (CK) of coronary issue were measured. Results: Perfusion with DPPH (0.25 mumol/L) for 5 min caused a significant impairment of cardiac function, as shown by a decrease in LVP and +dp/dtmax and an increase in LVEDP as well as the increased release of CK. Pretreatment with shenmai injection (1:320) for 10 min significantly improved the cardiac function and decreased the release of CK. Conclusion: Shenmai injection protects against the myocardial injury induced by free radical (DPPH) in isolated rabbit hearts.