Study on scavenging activity to DPPH free radical of different polarity components in Guizhou Miao medicine "bod zangd dak"
Du, H.-z.; Nong, H.; Dong, L.-s.; Li, J.-l.; Liu, M.; He, X.-c.; Zhang, J.
Zhongguo Zhong Yao Za Zhi 40(12): 2449-2454
2015
ISSN/ISBN: 1001-5302 PMID: 26591541 Document Number: 684661
The paper is aimed to search more natural plant antioxidants and further research and develop new medicinal plant resources in Guizhou. The Guizhou special miao medicine "bod zangd dak" was extracted with 60% ethanol. The antioxidant activity of the different polarity components separated from the extract was tested by DPPH method with ascorbic acid as positive control. The results showed that the IC50 of the different polarity components was as following: ascorbic acid (0.033 4 g x L(-1)) < ethyl acetate components (0.052 3 g x L(-1)) < total tannins components (0.054 9 g x L(-1)) < 60% ethanol extraction components (0.076 7 g x L(-1)) < butanol extraction components (0.110 g x L(-1)) < water-soluble polysaccharides components (0.168 g x L(-1)) < water extraction components (0.174 g x L(-1)) < water components after extraction (0.226 g x L(-1)) < total polysaccharides components (0.645 g x L(-1)). It is concluded that the different polarity components have different free radical scavenging activity and that provides a scientific basis for further search of the active ingredients and the activive mechanism.